Our services

Entering the field of extracellular vesicle (EV) research can be complex, particularly given the rapidly evolving methodologies and standards.

Whether you are initiating a new project or advancing existing studies, expert guidance is essential to ensure robust and reproducible outcomes.

Our EV consultation and support services provide tailored advice across all stages of research. This includes assistance with study design, methodological selection, and alignment with current best practices and international guidelines.

We work closely with researchers to optimise experimental approaches, enhance data quality, and maximise the impact of their EV studies.

EV isolation

Small volume EV isolation:

Our EV isolation for small volume samples (usually biofluids) is based on size-exclusion chromatography (SEC). We have established robust workflows for the isolation of highly purified EV preparations that retain their native structural morphology, biological function, and molecular composition, critical attributes for both mechanistic research and translational applications.

Large volume EV isolation:

Our EV isolation for large volume samples (recommended for cell culture media) is based on tangential flow filtration (TFF). Our TFF system can process samples up to 800 mL per run. The platform incorporates real-time process monitoring and an optimised workflow designed to maximise yield while preserving the biological activity of isolated EVs.

Cell line-specific EVs:

We also offer ready-to-use EVs from mesenchymal stem cells (adipose-derived MSCs), breast cancer, and HEK293 cells.

EV characterisation

Concentration and size determination:

Nanoparticle tracking analysis (NTA) provides particle size distribution profiles and concentration measurements, essential parameters for both quality control and downstream functional studies.

EV surface charge determination:

EV surface charge determination through zeta potential measurements.

EV protein concentration and purity assessment:

Micro bicinchoninic acid (BCA) protein quantification offers high sensitivity detection of total protein concentration in EV preparations, providing a critical quality control measure for sample purity and ensuring accurate, normalised protein loading for downstream Western blot analysis.

EV marker determination:

EV-optimised Western blot analysis of EV preparations for authentication and purity assessment. Detection of established EV-positive markers, including tetraspanins (CD81, CD9, CD63) and the endosomal-associated protein Alix, alongside negative contaminant markers, including calnexin (intracellular vesicle contamination) and apoA1/apoB (lipoprotein contamination).